DOI:10.1002/csc2.20026

Zhaoqiao Liu,Ting Liu,Linlin Liang, Zhou Li, Muhammad Jawad Hassan,Yan Peng, Duo Wang

发表期刊：Crop Science

链接：https://acsess.onlinelibrary.wiley.com/doi/10.1002/csc2.20026

Abstract：

Chitosan (CTS) is involved in regulating tolerance to abiotic stress in plants. Theobjectives of this study were to examine whether exogenous application of CTS couldimprove drought tolerance of creeping bentgrass (Agrostis stoloniferaL.) and revealCTS-regulated mechanisms of drought tolerance. Antioxidant enzyme activities, photosynthesis, and metabolic pathways of amino acids, carbohydrates, and energy production were measured. Plants pretreated with or without 100 mg CTS L⁻¹were subjected to well-watered or drought stress conditions for 14 d in growth chambers. Theincrease in endogenous CTS content by foliar spray of exogenous CTS significantlyimproved drought tolerance of creeping bentgrass as demonstrated by increased relative water content, cell membrane stability, photochemical efficiency, photosynthesis,and performance index on absorption basis under drought stress. The CTS applicationsignificantly reduced drought-caused oxidative damage by enhancing total antioxidantcapacity and activating superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase. During drought stress, pretreatment with CTS promoted water use efficiency,carbohydrate (sucrose and glucose) accumulation, energy production, and metabolichomeostasis. Chitosan treatment did not have an effect on proline accumulation andmetabolism. Improved γ-aminobutyric acid (GABA) accumulation, pyruvic acid, andchlorophyll content in leaves were found due to CTS treatment under drought stress.Results indicate that the CTS plays a positive role in regulating drought tolerance ofcreeping bentgrass by altering antioxidant, energy supply, and metabolic homeostasis.