DOI: 10.1186/s12870-020-02530-0

Min Sun, Dejun Huang,Ailing Zhang, Imran Khan,Haidong Yan, Xiaoshan Wang, Xinquan Zhang, Jian Zhang and Linkai Huang.

发表期刊：BMC Plantbiology

链接：https://www.researchgate.net/publication/340197713_Transcriptome_analysis_of_heat_stress_and_drought_stress_in_pearl_millet_based_on_Pacbio_full-length_transcriptome_sequencing

Abstract：

Background: Heat and drought are serious threats for crop growth and development. As the sixth largest cerealcrop in the world, pearl millet can not only be used for food and forage but also as a source of bioenergy. Pearmillet is highly tolerant to heat and drought.Given this it is considered an ideal crop to study plant stress toleranceand can be used to identify heat-resistant genes.

Results: In this study, we used Pacbio sequencing data as a reference sequence to analyze the llumina data of pearl millet that had been subjected to heat and drought stress for 48 h. By summarizing previous studies, we found 26,299 new genes and 63,090 new transcripts, and the number of gene annotations increased by 20.18%.Weidentified 2792 transcription factors and 1223 transcriptional regulators. There were 318 TFs and 149 TRs differentially expressed under heat stress, and 315 TFs and 128 TRs were differentially expressed under droughtstress. We used RNA sequencing to identify 6920 genes and 6484 genes differentially expressed under heat stressand drought stress, respectively.

Conclusions: Through Pacbio sequencing, we have identified more new genes and new transcripts. On the otherhand comparing the differentially expressed genes under heat tolerance with the DEGs under drought stress, wefound that even in the same pathway, pearl millet responds with a different protein.